资料上市这么说的：“DILUTION FACTOR= Concentration BEFORE dilution / Concentration AFTER dilution= Volume AFTER dilution / Volume BEFORE dilutionHenceConc. BEFORE dilution = Dilution factor x Conc. AFTER dilutione.g. If 5 ml of a solution was diluted to 100 ml the dilution factor would be 100 / 5 = 20 For multiple dilutions the dilution factor is the product of the dilution factors for each individual dilution.e.g. If 5 ml was diluted to 100 ml and 2 ml of the resulting solution was diluted to 50 ml the dilution factor would be:-(100 / 5) x (50 / 2) = 20 x 25 = 500From a practical point of view, the dilution factor for each step is:(Volume of flask) / (Volume of pipette)”所以我上面的问题中稀释因子应该是10/1=10，两种方法因该是第二种比较准确，不知理解是否正确？可是样品如果是固体是按重量计算，不是按体积计算怎么办？

我们是定容到10ml。就算是按重量计算，也可以算出结果来，没有用到稀释因子。

就稀释来说,当然是第二种方法符合梯度稀释的标准做法的(取1ml定容10ml),相当于稀释10倍.