国产麒麟拜耳的小涡旋振荡器，还有和你们一样的多管涡旋振荡器，好用；

pH变化了

看懂之后，对解决问题很有帮助

新柱子一定要老化后才能使用，不然会对柱子造成永久伤害虽然已经造成损害，还是试试看，柱头割掉一些，再试着老化一回，看看能不能用，不行只好换了

只有盐效应再起作用，没有同离子效应的

匀浆提取、震荡提取

0.3ml洗脱液是否能完全洗脱？

我是用左手，左手控制洗耳球不需要像右手那么大的灵活精确控制不过，也要看个人习惯吧，没所谓用左手或右手

气相的检出限是个难题呀

这里有个英文的，你看看硝基呋喃

不知道....

Monitoring of the residue of fosthiazate in water samples using solid-phase extraction coupled with gas chromatography/mass spectrometrySolid-phase extraction (SPE) coupled with gas chromatography/mass spectrometry (GC/MS) was used to determine the fosthiazate residue in water samples. The water samples were first filtered through cellulose filters (0.45 microm pore size). A 100 mL volume of filtered water, in which 1 mL of methanol has been added, was then passed through a pre-conditioned 3 cm C18 cartridge at a flow-rate of 1.5 mL/min. Elution was performed by 1 mL of methanol. The eluant was finally dried under reduced pressure for solvent evaporation. The volume was quantitatively adjusted to 0.5 mL with methanol. The analysis was carried out on GC/MS. The mass spectrometer was operated in selected ion monitoring (SIM) mode. According to mass spectrum of fosthiazate, three selected ions at m/z of 126, 195, 283, respectively, were monitored for identification and quantification. High sensitivity and selectivity were achieved by using this method. The limit of detection for fosthiazate in water samples was determined to be 56.4 ng/L. The linearity was demonstrated over a wide range of concentrations covering from 0.282 to 141 microg/L. The recoveries were more than 85.5% and the relative standard deviations for the overall procedure were less than 4.42%. The fosthiazate residue was detected in the water samples from a pool near cropland where fosthiazate was used. The results demonstrate the suitability of the SPE-GC/MS approach for the analysis of fosthiazate in water.

这个跟标准有关系

其实只要有合适的方法，什么兽药都能用酶标仪做，所以第一个问题意义不大；多残留用酶标做确实吃力，酶标的优点主要还是批量快速

白日做梦，用那玩意能做药残笑话吧

A liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination of disulfoton and five of its oxidative metabolites (disulfoton-sulfoxide, disulfoton-sulfone, demeton-S, demeton-S-sulfoxide and demeton-S-sulfone) in human whole blood and urine. Extraction was undertaken using a QuEChERS method, which is commonly used in food analysis. D10-Disulfoton was used as the internal standard. Separation was carried out using a CAPCELL-PAK MG II column (35×2.0 mm i.d., 5 μm, Shiseido) with a mobile phase of 10 m mol/L ammonium formate and methanol. This method was applied in an autopsy case, and disulfoton and its oxidative metabolites were successfully detected in both blood and urine. The concentrations of disulfoton in the blood and urine were 360 and 23.8 ng/mL, respectively. There was a relatively low concentration of demeton-S in both the blood (4.0 ng/mL) and urine (45.7 ng/mL). To date, there have been no reported cases of detection of demeton-S in human samples.

不错，以后类似的考试可以选些题目。

我们用LC-MS-MS

AB公司的产品质量确实是好，但调研中发现其维护保养等运行费用也很高，对于我们每年只检测一两百个样品（5年后可能会达到1000个）的小机构，负担可能过重

0.5ppb的回收率稍微低了点，1-10ppb的回收率不错，都在允许范围之内